A molecular risk score based on four functional pathways for advanced classical Hodgkin lymphoma
نویسندگان
چکیده
Despite improvement in the treatment of advanced classical Hodgkin Lymphoma, around 30% relapse or die as result of the disease. Current predictive systems, based on clinical and analytical parameters, fail to identify these high-risk patients accurately. We took a multistep approach to design a quantitative RT-PCR assay to be applied to routine formalin-fixed paraffin-embedded samples, integrating genes expressed either by the tumor cells and their microenvironment. The significance of 30 genes chosen on the basis of previously published data was evaluated in a set of 282 samples (divided into estimation and validation sets) to build a molecular risk score to predict failure. Adequate RT-PCR profiles were obtained from 262 of 282 cases (92.9%). Best predictor genes were integrated into an 11-gene model including four functional pathways (Cell Cycle, Apoptosis, Macrophage Activation and IRF4) able to identify lowand high-risk patients with different rates of 5-year FFS: 74% versus 44.1% in the estimation set (p < 0.0001); and 67.5% vs. 45.0% in the validation set (p = 0.0217). Moreover, this biological model can be combined with stage IV into a final predictive model able to identify a group of patients with very bad outcome (5-year FFS probability 25.2%). For personal use only. on October 28, 2017. by guest www.bloodjournal.org From
منابع مشابه
A molecular risk score based on 4 functional pathways for advanced classical Hodgkin lymphoma.
Despite improvement in the treatment of advanced classical Hodgkin lymphoma, approximately 30% of patients relapse or die as result of the disease. Current predictive systems, determined by clinical and analytical parameters, fail to identify these high-risk patients accurately. We took a multistep approach to design a quantitative reverse-transcription polymerase chain reaction assay to be app...
متن کاملDiagnosis and Treatment B non-Hodgkin Lymphoma with System Biology Approaches
Lymphomas are solid tumors of immune system and Non-Hodgkin Lymphomas (NHL) is the most prevalent lymphomas; with wide ranges of histological and clinical features, it is so difficult to identify them. Herein, various bioinformatics tools (such as gene differential expressions, epigenetics and protein analysis) employed to find new treatment approach for NHL based on gene expression variation b...
متن کاملNon-Hodgkin Lymphoma (NHL) in Pakistan
Lymphomas are classified as Hodgkin’s and Non-Hodgkin’s lymphomas (HL NHL) NHL being further sub-divided into B, T and Null cell categories on the basis of WHO classification. With a few exceptions worldwide, B-NHL are more common, accounting approximately 80-85% of all cases of NHL compared to T-NHL, which accounts for about 10-15% of all NHL cases. The incidence of NHL has shown a steady incr...
متن کاملLYMPHOID NEOPLASIA e-Blood A molecular risk score based on 4 functional pathways for advanced classical Hodgkin lymphoma
*Beatriz Sánchez-Espiridión,1 *Carlos Montalbán,2 Ángel López,1 Javier Menárguez,3 Pilar Sabín,3 Carmen Ruiz-Marcellán,4 Andrés Lopez,4 Rafael Ramos,5 Jose Rodríguez,5 Araceli Cánovas,6 Carmen Camarero,6 Miguel Canales,7 Javier Alves,7 Reyes Arranz,8 Agustín Acevedo,8 Antonio Salar,9 Sergio Serrano,9 Águeda Bas,10 Jose M. Moraleda,10 Pedro Sánchez-Godoy,11 Fernando Burgos,11 Concepción Rayón,12...
متن کاملPrognostic significance of Bcl-2, tumor-associated macrophages, and total neoplastic and inflammatory lymph node involvement in advanced stage classical Hodgkin's lymphoma.
BACKGROUND Although Hodgkin's lymphoma (HL) is a curable cancer, current treatment strategies based on risk stratification and response modulation are not precise enough. The predictive power of biological and morphological parameters is controversial, with prognostic models not reaching wide acceptance. PATIENTS AND METHODS We analyzed the prognostic relevance of 8 parameters in 85 advanced ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2010